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polya selection  (Illumina Inc)


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    Structured Review

    Illumina Inc polya selection
    Polya Selection, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 20574 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polya+selection/TruSeq+RNA+Library+Preparation+Kit+v2/pm41417109-195-27-19
    Average 99 stars, based on 20574 article reviews
    polya selection - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Produced:

    Article Title: Ecological genomics in the Northern krill uncovers loci for local adaptation across ocean basins.
    Article Snippet: .. NGI produced six libraries from the six K20 RNA extractions using Illumina TruSeq Stranded mRNA Library Prep kit with polyA selection (Illumina Cat# 20020594/5, manufacturers’ protocol #1000000040498), which were sequenced as 2 × 150bp paired-end reads on one Illumina NovaSeq 6000 SP lane. ..

    Selection:

    Article Title: Ecological genomics in the Northern krill uncovers loci for local adaptation across ocean basins.
    Article Snippet: .. NGI produced six libraries from the six K20 RNA extractions using Illumina TruSeq Stranded mRNA Library Prep kit with polyA selection (Illumina Cat# 20020594/5, manufacturers’ protocol #1000000040498), which were sequenced as 2 × 150bp paired-end reads on one Illumina NovaSeq 6000 SP lane. ..

    Article Title: RING1 missense variants reveal sensitivity of DNA damage repair to H2A monoubiquitination dosage during neurogenesis.
    Article Snippet: .. RNA library preparation was performed by Azenta Life Sciences with PolyA selection (Illumina). .. Libraries were sequenced on an Illumina 2x150bp instrument, targeting approximately 15million paired-end reads per sample.

    Article Title: Genetic determinants of endophytism in the Arabidopsis root mycobiome
    Article Snippet: .. Transcriptomes were sequenced using Illumina Truseq Stranded RNA protocols with polyA selection ( http://support.illumina.com/sequencing/sequencing_kits/truseq_stranded_mrna_ht_sample_prep_kit.html ) on HiSeq2500 using HiSeq TruSeq SBS sequencing kits v4 or NovaSeq6000 using NovaSeq XP v1 reagent kits, S4 flow cell, following a 2 × 150 indexed run recipe. ..

    Article Title: Spike protein-induced VSIR-ISX signaling disrupts metabolic homeostasis and promotes COVID-19-related immune dysfunction.
    Article Snippet: .. Next-generation sequencing (RNA-Seq) and data analysis Next-generation sequencing was performed as previously described (Wang et al. 2023b), using the Illumina TruSeq RNA Library Preparation Kit v2 with polyA selection to obtain 50 cycles of single-end reads. ..

    Article Title: RING1 missense variants reveal sensitivity of DNA damage repair to H2A monoubiquitination dosage during neurogenesis.
    Article Snippet: .. RNA library preparation was performed by performed by Azenta Life Sciences with PolyA selection (Illumina). .. Libraries were sequenced on an Illumina 2x150bp instrument, targeting approximately 15 million paired-end reads per sample.

    Article Title: BEHAVIORAL AND TRANSCRIPTOMIC EFFECTS OF A NOVEL CANNABINOID ON A RAT VALPROIC ACID MODEL OF AUTISM.
    Article Snippet: The 158 quality of the extracted RNA was determined by spectrophotometric analysis (NanoDrop) and high-159 J urn al Pr -pr oo f Page 9 of 23 resolution electrophoresis (Agilent Bioanalyzer). .. Library preparation proceeded with polyA selection 160 according to Illumina’s specifications. .. Strand-specific RNA sequencing (2x150bp) was performed on an 161 Illumina NovaSeq instrument, with a sequencing depth ~20M paired-end reads per sample.

    Article Title: Spike protein–induced VSIR–ISX signaling disrupts metabolic homeostasis and promotes COVID-19–related immune dysfunction
    Article Snippet: .. Next-generation sequencing was performed as previously described (Wang et al. ), using the Illumina TruSeq RNA Library Preparation Kit v2 with polyA selection to obtain 50 cycles of single-end reads. ..

    Article Title: DE-ETIOLATED1 has a role in the circadian clock of the liverwort Marchantia polymorpha.
    Article Snippet: RNA quality and quantity was analysed using an RNA 6000 Nano kit with a 2100 Bioanalyzer System (Agilent Technologies) before sequencing. .. Individual cDNA libraries for sequencing were prepared from 100 ng total RNA using the TruSeq stranded mRNA library preparation kit including polyA selection (Cat# 20020594/5, Illumina Inc.). ..

    RNA Library Preparation:

    Article Title: RING1 missense variants reveal sensitivity of DNA damage repair to H2A monoubiquitination dosage during neurogenesis.
    Article Snippet: .. RNA library preparation was performed by Azenta Life Sciences with PolyA selection (Illumina). .. Libraries were sequenced on an Illumina 2x150bp instrument, targeting approximately 15million paired-end reads per sample.

    Article Title: Spike protein-induced VSIR-ISX signaling disrupts metabolic homeostasis and promotes COVID-19-related immune dysfunction.
    Article Snippet: .. Next-generation sequencing (RNA-Seq) and data analysis Next-generation sequencing was performed as previously described (Wang et al. 2023b), using the Illumina TruSeq RNA Library Preparation Kit v2 with polyA selection to obtain 50 cycles of single-end reads. ..

    Article Title: RING1 missense variants reveal sensitivity of DNA damage repair to H2A monoubiquitination dosage during neurogenesis.
    Article Snippet: .. RNA library preparation was performed by performed by Azenta Life Sciences with PolyA selection (Illumina). .. Libraries were sequenced on an Illumina 2x150bp instrument, targeting approximately 15 million paired-end reads per sample.

    Article Title: Spike protein–induced VSIR–ISX signaling disrupts metabolic homeostasis and promotes COVID-19–related immune dysfunction
    Article Snippet: .. Next-generation sequencing was performed as previously described (Wang et al. ), using the Illumina TruSeq RNA Library Preparation Kit v2 with polyA selection to obtain 50 cycles of single-end reads. ..

    Sequencing:

    Article Title: Genetic determinants of endophytism in the Arabidopsis root mycobiome
    Article Snippet: .. Transcriptomes were sequenced using Illumina Truseq Stranded RNA protocols with polyA selection ( http://support.illumina.com/sequencing/sequencing_kits/truseq_stranded_mrna_ht_sample_prep_kit.html ) on HiSeq2500 using HiSeq TruSeq SBS sequencing kits v4 or NovaSeq6000 using NovaSeq XP v1 reagent kits, S4 flow cell, following a 2 × 150 indexed run recipe. ..

    Article Title: DE-ETIOLATED1 has a role in the circadian clock of the liverwort Marchantia polymorpha.
    Article Snippet: RNA quality and quantity was analysed using an RNA 6000 Nano kit with a 2100 Bioanalyzer System (Agilent Technologies) before sequencing. .. Individual cDNA libraries for sequencing were prepared from 100 ng total RNA using the TruSeq stranded mRNA library preparation kit including polyA selection (Cat# 20020594/5, Illumina Inc.). ..

    Next-Generation Sequencing:

    Article Title: Spike protein-induced VSIR-ISX signaling disrupts metabolic homeostasis and promotes COVID-19-related immune dysfunction.
    Article Snippet: .. Next-generation sequencing (RNA-Seq) and data analysis Next-generation sequencing was performed as previously described (Wang et al. 2023b), using the Illumina TruSeq RNA Library Preparation Kit v2 with polyA selection to obtain 50 cycles of single-end reads. ..

    Article Title: Spike protein–induced VSIR–ISX signaling disrupts metabolic homeostasis and promotes COVID-19–related immune dysfunction
    Article Snippet: .. Next-generation sequencing was performed as previously described (Wang et al. ), using the Illumina TruSeq RNA Library Preparation Kit v2 with polyA selection to obtain 50 cycles of single-end reads. ..



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    (A) Volcano plot analysis of host transcriptome-wide genes differentially expressed in primary B cells from three independent donors infected with WT or TES2m LMP1 for 21 days. Positive fold changes indicate higher transcripts in cells with TES2m LMP1. (B) PFKFB4 glucose metabolism roles. Glucose is metabolized by glycolysis or by the PPP. Glycolysis flux is regulated via Fructose-2,6-BP, an allosteric regulator of PFK1. PFKFB3 phosphorylates Fructose-6-P into Fructose-2,6-BP while PFKFB4 phosphatase activity converts Fructose-2,6-BP to Fructose-6-P to shunt glycolytic flux to PPP. Created in BioRender. Burton, E. (2026) https://BioRender.com/i0gawc8 . (C) KEGG pathway analysis of transcripts significantly upregulated in cells infected by EBV with WT LMP1 at day 21 post infection. (D) KEGG pathway analysis of transcripts significantly upregulated in cells infected by TES2m EBV at day 21 post infection. (E) PFKFB4 protein levels in WT versus TES2m LMP1 EBV infected cells. Cells from two independent donors were infected with EBV with WT LMP1 or TES2m LMP1 for 22 days. Densitometry values of PFKFB4 vs β-Actin load controls are shown. (F) Effects of LCL LMP1 KO on PFKFB4 <t>mRNA.</t> Median PFKFB4 reads from RNAseq analysis of Cas9+ GM12878 cells with control vs LMP1 targeting sgRNA expression for 48 hours. Shown are data from n=3 RNAseq datasets. (G) PFKFB4 protein levels in LMP1 KO LCLs. Cas9+ GM12878 were mock induced or induced to express LMP1 sgRNA for 48 hours. WCL was extracted and immunoblot was carried out using the indicated antibodies. (H) Effects of TES1 vs TES2 signaling inhibition on LCL PFKFB4 expression. Median PFKFB4 RNAseq reads from RNAseq analysis of Cas9+ GM12878 expressing LMP1 sgRNA together with dox-induced LMP1 TES1m vs WT LMP1 cDNA expression. Shown are data from n=3 RNAseq datasets. (I) Relative PFKFB4 protein abundances from proteomic analysis of primary human B-cells at the indicated days post-infection by the EBV B95.8 strain. Shown are mean ± SD values from n=4 replicates. (J) Effects of exogenous PFKFB4 expression on proliferation of primary B cells infected by EBV with WT versus TES2m LMP1. Peripheral blood B cells from three independent donors were infected with EBV with WT vs TES2m LMP1. After 14 days, cells were transduced with lentivirus control or with lentivirus driving stable PFKFB4 expression. Five days post-selection, cells were re-seeded. Twelve days later, live cell counts were defined. P-values were determined by one-sided Fisher’s exact test. * p<0.05, **p<0.005, ***p<0.0005. blots are representative of n=3 independent replicates.
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    (A) Volcano plot analysis of host transcriptome-wide genes differentially expressed in primary B cells from three independent donors infected with WT or TES2m LMP1 for 21 days. Positive fold changes indicate higher transcripts in cells with TES2m LMP1. (B) PFKFB4 glucose metabolism roles. Glucose is metabolized by glycolysis or by the PPP. Glycolysis flux is regulated via Fructose-2,6-BP, an allosteric regulator of PFK1. PFKFB3 phosphorylates Fructose-6-P into Fructose-2,6-BP while PFKFB4 phosphatase activity converts Fructose-2,6-BP to Fructose-6-P to shunt glycolytic flux to PPP. Created in BioRender. Burton, E. (2026) https://BioRender.com/i0gawc8 . (C) KEGG pathway analysis of transcripts significantly upregulated in cells infected by EBV with WT LMP1 at day 21 post infection. (D) KEGG pathway analysis of transcripts significantly upregulated in cells infected by TES2m EBV at day 21 post infection. (E) PFKFB4 protein levels in WT versus TES2m LMP1 EBV infected cells. Cells from two independent donors were infected with EBV with WT LMP1 or TES2m LMP1 for 22 days. Densitometry values of PFKFB4 vs β-Actin load controls are shown. (F) Effects of LCL LMP1 KO on PFKFB4 <t>mRNA.</t> Median PFKFB4 reads from RNAseq analysis of Cas9+ GM12878 cells with control vs LMP1 targeting sgRNA expression for 48 hours. Shown are data from n=3 RNAseq datasets. (G) PFKFB4 protein levels in LMP1 KO LCLs. Cas9+ GM12878 were mock induced or induced to express LMP1 sgRNA for 48 hours. WCL was extracted and immunoblot was carried out using the indicated antibodies. (H) Effects of TES1 vs TES2 signaling inhibition on LCL PFKFB4 expression. Median PFKFB4 RNAseq reads from RNAseq analysis of Cas9+ GM12878 expressing LMP1 sgRNA together with dox-induced LMP1 TES1m vs WT LMP1 cDNA expression. Shown are data from n=3 RNAseq datasets. (I) Relative PFKFB4 protein abundances from proteomic analysis of primary human B-cells at the indicated days post-infection by the EBV B95.8 strain. Shown are mean ± SD values from n=4 replicates. (J) Effects of exogenous PFKFB4 expression on proliferation of primary B cells infected by EBV with WT versus TES2m LMP1. Peripheral blood B cells from three independent donors were infected with EBV with WT vs TES2m LMP1. After 14 days, cells were transduced with lentivirus control or with lentivirus driving stable PFKFB4 expression. Five days post-selection, cells were re-seeded. Twelve days later, live cell counts were defined. P-values were determined by one-sided Fisher’s exact test. * p<0.05, **p<0.005, ***p<0.0005. blots are representative of n=3 independent replicates.
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    Image Search Results


    (A) Volcano plot analysis of host transcriptome-wide genes differentially expressed in primary B cells from three independent donors infected with WT or TES2m LMP1 for 21 days. Positive fold changes indicate higher transcripts in cells with TES2m LMP1. (B) PFKFB4 glucose metabolism roles. Glucose is metabolized by glycolysis or by the PPP. Glycolysis flux is regulated via Fructose-2,6-BP, an allosteric regulator of PFK1. PFKFB3 phosphorylates Fructose-6-P into Fructose-2,6-BP while PFKFB4 phosphatase activity converts Fructose-2,6-BP to Fructose-6-P to shunt glycolytic flux to PPP. Created in BioRender. Burton, E. (2026) https://BioRender.com/i0gawc8 . (C) KEGG pathway analysis of transcripts significantly upregulated in cells infected by EBV with WT LMP1 at day 21 post infection. (D) KEGG pathway analysis of transcripts significantly upregulated in cells infected by TES2m EBV at day 21 post infection. (E) PFKFB4 protein levels in WT versus TES2m LMP1 EBV infected cells. Cells from two independent donors were infected with EBV with WT LMP1 or TES2m LMP1 for 22 days. Densitometry values of PFKFB4 vs β-Actin load controls are shown. (F) Effects of LCL LMP1 KO on PFKFB4 mRNA. Median PFKFB4 reads from RNAseq analysis of Cas9+ GM12878 cells with control vs LMP1 targeting sgRNA expression for 48 hours. Shown are data from n=3 RNAseq datasets. (G) PFKFB4 protein levels in LMP1 KO LCLs. Cas9+ GM12878 were mock induced or induced to express LMP1 sgRNA for 48 hours. WCL was extracted and immunoblot was carried out using the indicated antibodies. (H) Effects of TES1 vs TES2 signaling inhibition on LCL PFKFB4 expression. Median PFKFB4 RNAseq reads from RNAseq analysis of Cas9+ GM12878 expressing LMP1 sgRNA together with dox-induced LMP1 TES1m vs WT LMP1 cDNA expression. Shown are data from n=3 RNAseq datasets. (I) Relative PFKFB4 protein abundances from proteomic analysis of primary human B-cells at the indicated days post-infection by the EBV B95.8 strain. Shown are mean ± SD values from n=4 replicates. (J) Effects of exogenous PFKFB4 expression on proliferation of primary B cells infected by EBV with WT versus TES2m LMP1. Peripheral blood B cells from three independent donors were infected with EBV with WT vs TES2m LMP1. After 14 days, cells were transduced with lentivirus control or with lentivirus driving stable PFKFB4 expression. Five days post-selection, cells were re-seeded. Twelve days later, live cell counts were defined. P-values were determined by one-sided Fisher’s exact test. * p<0.05, **p<0.005, ***p<0.0005. blots are representative of n=3 independent replicates.

    Journal: bioRxiv

    Article Title: Epstein-Barr Virus Latent Membrane Protein 1 Suppresses Ferroptosis via Pentose Phosphate Pathway and Glutathione Metabolism

    doi: 10.64898/2026.03.09.710628

    Figure Lengend Snippet: (A) Volcano plot analysis of host transcriptome-wide genes differentially expressed in primary B cells from three independent donors infected with WT or TES2m LMP1 for 21 days. Positive fold changes indicate higher transcripts in cells with TES2m LMP1. (B) PFKFB4 glucose metabolism roles. Glucose is metabolized by glycolysis or by the PPP. Glycolysis flux is regulated via Fructose-2,6-BP, an allosteric regulator of PFK1. PFKFB3 phosphorylates Fructose-6-P into Fructose-2,6-BP while PFKFB4 phosphatase activity converts Fructose-2,6-BP to Fructose-6-P to shunt glycolytic flux to PPP. Created in BioRender. Burton, E. (2026) https://BioRender.com/i0gawc8 . (C) KEGG pathway analysis of transcripts significantly upregulated in cells infected by EBV with WT LMP1 at day 21 post infection. (D) KEGG pathway analysis of transcripts significantly upregulated in cells infected by TES2m EBV at day 21 post infection. (E) PFKFB4 protein levels in WT versus TES2m LMP1 EBV infected cells. Cells from two independent donors were infected with EBV with WT LMP1 or TES2m LMP1 for 22 days. Densitometry values of PFKFB4 vs β-Actin load controls are shown. (F) Effects of LCL LMP1 KO on PFKFB4 mRNA. Median PFKFB4 reads from RNAseq analysis of Cas9+ GM12878 cells with control vs LMP1 targeting sgRNA expression for 48 hours. Shown are data from n=3 RNAseq datasets. (G) PFKFB4 protein levels in LMP1 KO LCLs. Cas9+ GM12878 were mock induced or induced to express LMP1 sgRNA for 48 hours. WCL was extracted and immunoblot was carried out using the indicated antibodies. (H) Effects of TES1 vs TES2 signaling inhibition on LCL PFKFB4 expression. Median PFKFB4 RNAseq reads from RNAseq analysis of Cas9+ GM12878 expressing LMP1 sgRNA together with dox-induced LMP1 TES1m vs WT LMP1 cDNA expression. Shown are data from n=3 RNAseq datasets. (I) Relative PFKFB4 protein abundances from proteomic analysis of primary human B-cells at the indicated days post-infection by the EBV B95.8 strain. Shown are mean ± SD values from n=4 replicates. (J) Effects of exogenous PFKFB4 expression on proliferation of primary B cells infected by EBV with WT versus TES2m LMP1. Peripheral blood B cells from three independent donors were infected with EBV with WT vs TES2m LMP1. After 14 days, cells were transduced with lentivirus control or with lentivirus driving stable PFKFB4 expression. Five days post-selection, cells were re-seeded. Twelve days later, live cell counts were defined. P-values were determined by one-sided Fisher’s exact test. * p<0.05, **p<0.005, ***p<0.0005. blots are representative of n=3 independent replicates.

    Article Snippet: To construct indexed libraries, 1 μg of total RNA was used for polyA mRNA selection using NEBNext Poly(A) mRNA Magnetic Isolation Module (Cat#E7490S), and library preparation with NEBNext Ultra RNA Library Prep with Sample Purification Beads (Cat#E7765S).

    Techniques: Infection, Activity Assay, RNA sequencing, Control, Expressing, Western Blot, Inhibition, Transduction, Selection